Keywords: Dickeya Pectobacterium rot bacteriosis banana characterizationīanana ( Musa sp.) is an herbaceous plant belonging to the Musaceae family. This is valuable information for the implementation of appropriate disease management practices in the area. It was concluded that rhizome and pseudostem wet rot of banana grown organically in Piura is caused by the bacterial complex formed by P. carotovorum isolates 03MB and 08MB corresponded to Dickeya chrysanthemi and 02MB and 07MB corresponded to Dickeya paradisiaca and Klebsiella variicola, respectively. Molecular identification and phylogenetic analysis confirmed that isolates 01MB, 04MB, 05MB and 10MB corresponded to Pectobacterium carotovorum subsp. Isolates 02MB, 03MB, 08MB, 07MB and 09MB also tested positive for erythromycin and indole, while isolates 01MB, 04MB, 05MB, 06MB and 10MB were positive for CVP (Crystal violet pectate).
BANANA PLANT STEM ROT MAC
The biochemical tests showed that all the isolates were positive on nutrient agar medium, Mac Conkey, catalase, gelatin hydrolysis and growth at 37☌, whereas they were negative on King’s B medium, nitrate reductase and oxidase tests.
The 10 isolates were gram negative and with perimeter flagella. The pathogenicity of the isolates was confirmed by in vitro and in vivo inoculation of rhizome pieces and pseudostems from cultivated plants. Sixty colonies were obtained, while 10 isolates were selected for presenting cultural and morphological characteristics similar to those of Pectobacterium (01MB, 04MB, 05MB, 06MB and 10MB) and Dickeya (02MB, 03MB, 08MB, 07MB and 09MB) different biochemical and molecular tests were conducted. Bacterial isolates were extracted from rhizome and pseudostem samples of organic bananas with typical symptoms of soft rot from different banana growing areas of Piura. The aim of this research was to characterize the causal agent of banana bacteriosis disease in Piura. The cultivation of the crop is mainly affected by biotic factors, resulting in important economic losses. Organic banana ( Musa sp.) is one of the most important export crops in Peru, especially in the Department of Piura.
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